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human coronary endothelial cells hcaecs  (ATCC)


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    ATCC human coronary endothelial cells hcaecs
    MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected <t>HCAECs</t> was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
    Human Coronary Endothelial Cells Hcaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+coronary+arterial+endothelial+cells+hcaecs/Primary+Coronary+Artery+Endothelial+Cells%3B+Normal%2C+Human/pmc13167943-36-0-8
    Average 95 stars, based on 158 article reviews
    human coronary endothelial cells hcaecs - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy"

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    Journal: Frontiers in Pediatrics

    doi: 10.3389/fped.2026.1731155

    MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
    Figure Legend Snippet: MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Techniques Used: Expressing, Infection, Quantitative RT-PCR, Western Blot

    UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.
    Figure Legend Snippet: UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Techniques Used: Infection, Quantitative RT-PCR, Expressing, Western Blot

    UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
    Figure Legend Snippet: UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Techniques Used: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
    Figure Legend Snippet: MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Techniques Used: Infection, Quantitative RT-PCR, Expressing, Western Blot

    MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
    Figure Legend Snippet: MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Techniques Used: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
    Figure Legend Snippet: MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Techniques Used: Expressing, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay

    Related Articles

    Expressing:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.

    Derivative Assay:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.

    Infection:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.

    Real-time Polymerase Chain Reaction:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.

    Produced:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.

    Control:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.

    Western Blot:

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. HCAEC culture was performed according to the manufacturer’s instructions.HCAEC culture was performed according to the manufacturer’s instructions.

    Article Title: Anti-inflammatory effect of resveratrol in human coronary arterial endothelial cells via induction of autophagy: implication for the treatment of Kawasaki disease
    Article Snippet: Human coronary arterial endothelial cells (HCAECs) were obtained from the American Type Culture Collection and maintained at 37 °C under humidified 5% CO 2 in a stationary culture.. HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.HCAECs were cultured in Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), and used at passages two or three.



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    Image Search Results


    MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot

    UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

    UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

    MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Expressing, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay